TTUHSC SOM Cancer Center Core Laboratories
Flow Cytometry Core This core has two instruments:
BD LSRII This is a multi-laser 13 color analyzer. Users who have completed adequate training can operate this instrument.
BD FACS Aria This is a multi-laser sorter equipped with a 96 well autoclone device. Access to the sorter is strictly via the core technician.
Recharges: Currently no recharges are imposed, but use of instruments must be approved by the Cancer Center Director or his designee.
Contact for the core: Malkanthi Mudannayake, PhD m.mudannayake@ttuhsc.edu
Molecular Biology Core
Quantitative RT-PCR An ABI TaqMan instrument is available. The TaqMan is equipped to run TaqMan low density arrays.
DNA Sequencer An ABI sequencer optimized for Short Tandem Repeat assays (STR) provides robust cell line identification. At this time routine sequencing is not done on this instrument.
Recharges: Currently no recharges are imposed, but use of instruments must be approved by the Cancer Center Director or his designee.
Contact for the core: Taylor Chen, PhD taylor.chen@TTUHSC.edu
Immunocompromised Mouse Core
The Cancer Center maintains a laminar flow caging system in the TTUHSC Lubbock animal facility. Access to cancer investigators is available but space is limited. For more information contact C Patrick Reynolds, MD PhD.
Contact for the core: Pat Frisbie Patricia.Frisbie@TTUHSC.edu
Clinical Pharmacology Core
This core supports oncology clinical trials and also limited preclinical studies (i.e. those leading directly to a clinical trial). The core is equipped with a tandem mass spectrometer (ABI 4000 Q Trap) and four HPLC systems.
Contact for the core: Min Kang, PharmD min.kang@ttuhsc.edu
Cell Culture Core
This core laboratory serves as the Cell Culture and Xenograft Repository (www.COGcell.org) for the Childen's Oncology Group and also for the South Plains Oncology Consortium. New cell lines and xenografts are initiated from both adults and children with cancer, expanded, characterized, and readied for distribution. The core can also provide cell lines that are obtained from other sources (which can be subdistributed) to TTUHSC investigators. All cell lines are tested for mycoplasma and cell line identity verified by STR. Storage of "back up" cryopreserved samples is available for TTUHSC investigators.
Contact for the core: Tito Woodburn, tito.woodburn@ttuhsc.edu
Cell Line Identification
Methods for Authentication of Human Cell Cultures and Tissues
